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Pipette Calibration: The Gravimetric Method and Balance Resolution

By Ann Lenhardt & Erin Lenhardt

The most common and economical method used for pipette calibration is gravimetric analysis, defined as the quantitative determination of  an analyte based on the mass of a solid. Many internationally known standards such as the ISO 8655 Part 6: Gravimetric Methods for the Determination of Measurement Error and the ASTM E-11154-89 Standard Specification for Piston or Plunger Operated Volumetric Apparatus are built upon the gravimetric method of calibration; accordingly, most accredited ISO 17025 calibration laboratories use this method.

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